Journal: Applied and Environmental Microbiology
Article Title: A recombineering-based platform for high-throughput genomic editing in Escherichia coli
doi: 10.1128/aem.00193-25
Figure Lengend Snippet: Large-fragment deletion in E. coli MG1655 and E. coli Nissle 1917. ( A ) Genomic editing tests of sequence deletion of different lengths in MG1655. ( B ) E. coli MG1655 genomic editing results of sequence deletion of different sizes. ( C ) Deletion of E. coli MG1655 large fragments in different genomic locations. ( D ) Genomic editing tests of sequence deletion of different lengths in EcN. ( E ) EcN genomic editing results of sequence deletion of different sizes. (F through H) The sequencing results of 10 kb, 22 kb, and 58 kb fragment deletions.
Article Snippet: E. coli strains JM109 (ATCC 53323), MG1655 (ATCC 47076), JW128 , Nissle 1917 , and DH10B served as genetic materials in genomic editing experiments.
Techniques: Sequencing